User:Michael F. Nagle/Notebook/Chem 571/2013/02/12

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Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010001
50001508507501
50001009005001
5000409602001
500016984801
50006.4993.6321

This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.

[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.50.020.0033310000.62002.383
0.50.020.003337500.61502.383
0.50.020.003335000.61002.383
0.50.020.003332000.6402.383
0.50.020.00333800.6162.383
0.50.020.00333320.66.42.383

Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.000116022
1500.000192818
1000.000139227
405.55556E-06
166.68508E-05
6.40.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0058619.047619
0.0066666675186.246418
0.017182.539683
0.025180000
0.062514958.67769
0.1562525641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.00021989
1500.000147514
1000.000144751
400.00010884
168.12155E-05
6.43.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0054547.738693
0.0066666676779.026217
0.016908.396947
0.0259187.817259
0.062512312.92517
0.1562525857.14286



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