User:Michael F. Nagle/Notebook/Chem 571/2013/02/13

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==Description==
 
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*Calculations using all three assays were performed:
 
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# Velocity by Concentration was graphed for each run
 
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# An average velocity of all three runs was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for each run and Km was calculated
 
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# A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
 
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# Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
 
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*Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
 
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# An Average velocity of combine data was graphed by concentration
 
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# A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
 
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# Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values
 
==Data==
==Data==

Revision as of 16:27, 8 May 2013

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Data

  • All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm)
2000.0001803850.0055543.691027
1500.0001837940.0066666675440.881764
1000.0001626780.016147.11077
400.0001119810.0258930.093256
167.95434E-050.062512571.75005
6.43.42451E-050.1562529201.27683

Image:Combined_Average_Velocity_over_COncentration.png

Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png


  • Km calculations
Equation Value calculated
(-1)/Km0.028053516
Km35.64615572
1/Vmax4440.372695
Vmax0.000225206

Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin32 uM
Kaempferol32 uM
Quericetin26 uM



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