User:Michael F. Nagle/Notebook/Chem 571/2013/02/13

(Difference between revisions)
 Revision as of 16:27, 8 May 2013 (view source) (→Objective)← Previous diff Revision as of 16:27, 8 May 2013 (view source) (→Description)Next diff → Line 8: Line 8: - ==Description== - *Calculations using all three assays were performed: - # Velocity by Concentration was graphed for each run - # An average velocity of all three runs was graphed by concentration - # A Lineweaver-Burke Plot was graphed for each run and Km was calculated - # A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated - # Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values - *Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs - # An Average velocity of combine data was graphed by concentration - # A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated - # Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values ==Data== ==Data==

Revision as of 16:27, 8 May 2013

Project name Main project page
Previous entry      Next entry

Data

• All Graphs and Tables are for the Combined Data:
 Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm) 200 0.000180385 0.005 5543.691027 150 0.000183794 0.006666667 5440.881764 100 0.000162678 0.01 6147.11077 40 0.000111981 0.025 8930.093256 16 7.95434E-05 0.0625 12571.75005 6.4 3.42451E-05 0.15625 29201.27683

• Km calculations
 Equation Value calculated (-1)/Km 0.028053516 Km 35.64615572 1/Vmax 4440.372695 Vmax 0.000225206

Notes

• For run #2 concentration 40 uM was removed as an outlier
• For run #1 no values for concentration 150 uM were used
• Ki values for the inhibitors were found in a paper from Planta Medica:
 Flavanoid Ki value) Myricetin 32 uM Kaempferol 32 uM Quericetin 26 uM