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| ==Objective==
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| Use data collected yesterday to calculate Average values of the Assay and calculate Km
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| ==Description==
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| *Calculations using all three assays were performed:
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| # Velocity by Concentration was graphed for each run
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| # An average velocity of all three runs was graphed by concentration
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| # A Lineweaver-Burke Plot was graphed for each run and Km was calculated
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| # A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
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| # Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
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| *Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
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| # An Average velocity of combine data was graphed by concentration
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| # A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
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| # Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values
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| ==Data==
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| * All Graphs and Tables are for the Combined Data:
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Final Concentration Adenosine (uM)'''
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| | align="center" style="background:#f0f0f0;"|'''Average velocity over 180 seconds (nm/sec)'''
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| | align="center" style="background:#f0f0f0;"|'''1/[Adenosine] (1/uM)'''
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| | align="center" style="background:#f0f0f0;"|'''1/average velocity (sec/nm)'''
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| |-
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| | 200||0.000180385||0.005||5543.691027
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| |-
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| | 150||0.000183794||0.006666667||5440.881764
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| |-
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| | 100||0.000162678||0.01||6147.11077
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| |-
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| | 40||0.000111981||0.025||8930.093256
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| |-
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| | 16||7.95434E-05||0.0625||12571.75005
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| |-
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| | 6.4||3.42451E-05||0.15625||29201.27683
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| |}
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| [[Image:Combined_Average_Velocity_over_COncentration.png]]
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| [[Image:COMBINATION_AVERAGE_LINEWEAKVER-BURKEPLOT.png]]
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| * Km calculations
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Equation'''
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| | align="center" style="background:#f0f0f0;"|'''Value calculated'''
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| |-
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| | (-1)/Km||0.028053516
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| |-
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| | Km||35.64615572
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| |-
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| | 1/Vmax||4440.372695
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| |-
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| | Vmax||0.000225206
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| |}
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| ==Notes==
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| * For run #2 concentration 40 uM was removed as an outlier
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| * For run #1 no values for concentration 150 uM were used
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| * Ki values for the inhibitors were found in a paper from Planta Medica:
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| {| {{table}}
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| | align="center" style="background:#f0f0f0;"|'''Flavanoid'''
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| | align="center" style="background:#f0f0f0;"|'''Ki value)'''
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| |-
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| | Myricetin||32 uM
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| |-
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| | Kaempferol||32 uM
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| |-
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| | Quericetin||26 uM
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| |}
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