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Project name
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Objective
Use data collected yesterday to calculate Average values of the Assay and calculate Km
Description
- Calculations using all three assays were performed:
- Velocity by Concentration was graphed for each run
- An average velocity of all three runs was graphed by concentration
- A Lineweaver-Burke Plot was graphed for each run and Km was calculated
- A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
- Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
- Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
- An Average velocity of combine data was graphed by concentration
- A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
- Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values
Data
- All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM)
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Average velocity over 180 seconds (nm/sec)
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1/[Adenosine] (1/uM)
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1/average velocity (sec/nm)
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200 |
0.000180385 |
0.005 |
5543.691027
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150 |
0.000183794 |
0.006666667 |
5440.881764
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100 |
0.000162678 |
0.01 |
6147.11077
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40 |
0.000111981 |
0.025 |
8930.093256
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16 |
7.95434E-05 |
0.0625 |
12571.75005
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6.4 |
3.42451E-05 |
0.15625 |
29201.27683
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Equation
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Value calculated
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(-1)/Km |
0.028053516
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Km |
35.64615572
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1/Vmax |
4440.372695
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Vmax |
0.000225206
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Notes
- For run #2 concentration 40 uM was removed as an outlier
- For run #1 no values for concentration 150 uM were used
- Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid
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Ki value)
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Myricetin |
32 uM
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Kaempferol |
32 uM
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Quericetin |
26 uM
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