User:Michael F. Nagle/Notebook/Chem 571/2013/02/27: Difference between revisions

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(Autocreate 2013/02/27 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
 
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==Entry title==
==Procedure==
* Insert content here...
*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.  
**The following concentrations were used.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
|-
| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
|-
| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
|-
| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
|-
| 18.87||7.45856E-05||0.052994171||13407.40741||||||
|-
| 25.64||8.67403E-05||0.03900156||11528.66242||||||
|-
| 40||9.94475E-05||0.025||10055.55556||||||
|-
|
|}


#
*10mM Quercetin stock was prepared.
*Cuvettes with the following solutions were prepared and analyzed.
**Today, we only had time to complete the first two.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
| align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
|-
| 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
|-
| 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
|-
| 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
|-
| 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
|-
| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
|-
|
|}


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|}
|}
==Data==
*The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
[[Image:3_5data.JPG]]
*Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.


__NOTOC__
__NOTOC__

Revision as of 11:47, 5 March 2013

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Procedure

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.52 3.70166E-05 0.095057034 27014.92537 Km 44.67191792
12.34 5.13812E-05 0.081037277 19462.36559 1/Vmax 2662.131337
14.93 6.0221E-05 0.066979236 16605.50459 Vmax 0.000375639
18.87 7.45856E-05 0.052994171 13407.40741
25.64 8.67403E-05 0.03900156 11528.66242
40 9.94475E-05 0.025 10055.55556
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.5 0.02 0.00333 52.63157895 0.6 10.52631579 1.78 0.6 65 13 3
0.5 0.02 0.00333 61.72839506 0.6 12.34567901 1.78 0.6 65 13 3
0.5 0.02 0.00333 74.62686567 0.6 14.92537313 1.78 0.6 65 13 3
0.5 0.02 0.00333 94.33962264 0.6 18.86792453 1.78 0.6 65 13 3
0.5 0.02 0.00333 128.2051282 0.6 25.64102564 1.78 0.6 65 13 3
0.5 0.02 0.00333 200 0.6 40 1.78 0.6 65 13 3

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.




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