User:Michael F. Nagle/Notebook/Chem 571/2013/02/27

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(Autocreate 2013/02/27 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
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==Entry title==
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==Procedure==
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* Insert content here...
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*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.  
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**The following concentrations were used.
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
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| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
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| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
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| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
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|-
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| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
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|-
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| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
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|-
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| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
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|-
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| 18.87||7.45856E-05||0.052994171||13407.40741||||||
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|-
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| 25.64||8.67403E-05||0.03900156||11528.66242||||||
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|-
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| 40||9.94475E-05||0.025||10055.55556||||||
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|-
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|
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|}
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#
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*10mM Quercetin stock was prepared.
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*Cuvettes with the following solutions were prepared and analyzed.
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**Today, we only had time to complete the first two.
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 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
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| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
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| align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
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|-
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| 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
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|-
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| 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
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|-
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| 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
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|-
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| 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
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|-
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| 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
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|-
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| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
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|-
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|
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|}
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|}
|}
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==Data==
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*The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
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[[Image:3_5data.JPG]]
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*Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.
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 +
 +
 +
__NOTOC__
__NOTOC__

Revision as of 13:47, 5 March 2013

Project name Main project page
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Procedure

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.523.70166E-050.09505703427014.92537Km44.67191792
12.345.13812E-050.08103727719462.365591/Vmax2662.131337
14.936.0221E-050.06697923616605.50459Vmax0.000375639
18.877.45856E-050.05299417113407.40741
25.648.67403E-050.0390015611528.66242
409.94475E-050.02510055.55556
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.50.020.0033352.631578950.610.526315791.780.665133
0.50.020.0033361.728395060.612.345679011.780.665133
0.50.020.0033374.626865670.614.925373131.780.665133
0.50.020.0033394.339622640.618.867924531.780.665133
0.50.020.00333128.20512820.625.641025641.780.665133
0.50.020.003332000.6401.780.665133

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

Image:3_5data.JPG

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.




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