User:Michael F. Nagle/Notebook/Chem 571/2013/02/27

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**Today, we only had time to complete the first two.
**Today, we only had time to complete the first two.
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==Data==
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*The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
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==Data==
 
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*The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
 
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__NOTOC__
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Revision as of 15:19, 5 March 2013

Project name Main project page
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Procedure

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.50.020.0033352.631578950.610.526315791.780.665133
0.50.020.0033361.728395060.612.345679011.780.665133
0.50.020.0033374.626865670.614.925373131.780.665133
0.50.020.0033394.339622640.618.867924531.780.665133
0.50.020.00333128.20512820.625.641025641.780.665133
0.50.020.003332000.6401.780.665133
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.


Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.523.70166E-050.09505703427014.92537Km44.67191792
12.345.13812E-050.08103727719462.365591/Vmax2662.131337
14.936.0221E-050.06697923616605.50459Vmax0.000375639
18.877.45856E-050.05299417113407.40741
25.648.67403E-050.0390015611528.66242
409.94475E-050.02510055.55556

Image:3_5data.JPG

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.




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