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| Line 6: |
Line 6: |
| | colspan="2"| | | | colspan="2"| |
| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Procedure==
| |
| *Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
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| **The following concentrations were used.
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| {| {{table}}
| |
| | align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
| |
| |-
| |
| | 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
| |
| |-
| |
| |
| |
| |}
| |
|
| |
| *10mM Quercetin stock was prepared.
| |
| *Cuvettes with the following solutions were prepared and analyzed.
| |
| **Today, we only had time to complete the first two.
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|
Project name
