User:Michael S. Bible/Notebook/571/2014/09/09

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Objective

Today we took UV/Vis spectra of the amino acid solutions made on 9/3.

Description

  1. Add experimental record here. Include what, how, and why...

UV-Vis Absorbance Curves for Amino Acids

Fluorometry

Below is a graph of the data acquired from fluorometry on 81.2 nM L-Tryptophan. A wavelength of 275 nm was used to excite the solution. The small molarity is the result of a 2500:1 dilution of a 203 μM solution of L-Tryptophan. Tryptophan was the only one of our 5 amino acids that produced any significant results in UV-Vis and was, therefore, the only amino acid that was tested using Fluorometry.