User:Michael S. Bible/Notebook/571/2014/09/16: Difference between revisions

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==Description==
==Description==


*Today we dialyzed a 35:1 Au:Protein solution made earlier and stored in our lab drawer.
*Today we dialyzed a 35:1 Au:Protein solution (colloid solution) made earlier and stored in our lab drawer.
*For dialysis, we needed to create 1 L of a 50 mM Glycine buffer, with a pH ~3.5.
**To do this, 4 mL of 1 M HCl and 3.7608 grams of glycine were combined in a 1 L volumetric flask.
*We also needed to prepare 500 mL of a 50 mM NaCl solution. 
**To do this, 1.46307 g of NaCl was added to water in a 500 mL volumetric flask. 
*Additionally, 25 mL of 1 g/L Lysozyme was prepared.
**To do this, 0.025 g of Lysozyme was combined with water in a 25 mL volumetric flask. 
 
*We then performed dialysis using the protocol described in the "Dialysis Protocol" section below to prepare the dialysis tubes. 
**Dialysis was performed by adding 4 mL of the lysozyme solution to:
***A 3500 g/mol MWCO tube in NaCl soak.
***A 3500 g/mol MWCO tube in Glycine Buffer soak.
***A 3500 g/mol MWCO tube in HPLC water soak.
***A 25000 g/mol MWCO tube in Glycine Buffer soak.
**An additional dialysis was performed using 4 mL of the colloid solution in:
***A 3500 g/mol MWCO tube in Glycine Buffer soak.


==Data==
==Data==

Revision as of 15:19, 17 September 2014

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Dialysis of Colloid Sample

Protocols for today were adopted from Dr. Fox's Notebook.

Description

  • Today we dialyzed a 35:1 Au:Protein solution (colloid solution) made earlier and stored in our lab drawer.
  • For dialysis, we needed to create 1 L of a 50 mM Glycine buffer, with a pH ~3.5.
    • To do this, 4 mL of 1 M HCl and 3.7608 grams of glycine were combined in a 1 L volumetric flask.
  • We also needed to prepare 500 mL of a 50 mM NaCl solution.
    • To do this, 1.46307 g of NaCl was added to water in a 500 mL volumetric flask.
  • Additionally, 25 mL of 1 g/L Lysozyme was prepared.
    • To do this, 0.025 g of Lysozyme was combined with water in a 25 mL volumetric flask.
  • We then performed dialysis using the protocol described in the "Dialysis Protocol" section below to prepare the dialysis tubes.
    • Dialysis was performed by adding 4 mL of the lysozyme solution to:
      • A 3500 g/mol MWCO tube in NaCl soak.
      • A 3500 g/mol MWCO tube in Glycine Buffer soak.
      • A 3500 g/mol MWCO tube in HPLC water soak.
      • A 25000 g/mol MWCO tube in Glycine Buffer soak.
    • An additional dialysis was performed using 4 mL of the colloid solution in:
      • A 3500 g/mol MWCO tube in Glycine Buffer soak.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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