User:Michael S. Bible/Notebook/571/2014/09/23: Difference between revisions
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[[Image:Bradford_UV_VIS_Chart.png|500px]] | [[Image:Bradford_UV_VIS_Chart.png|500px]] | ||
[[Image: | [[Image:Bradford_Calibration_Curve_9-23.png|500px]] | ||
==Notes== | ==Notes== |
Revision as of 10:55, 1 October 2014
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Objective
DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
The following graphs were produced using UV-Vis data collected analysis of each of the solutions above. It was observed that at concentrations above 20 μg/mL the calibration curve is no longer linear, so the second graph only includes concentrations of lysozyme < 20 μg/mL. NotesBradford Assay is only effective from 1μg/mL to 10μg/mL
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