User:Michael S. Bible/Notebook/571/2014/09/30: Difference between revisions
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==Description== | ==Description== | ||
#Dialysis | |||
#*Prepared 10 mL 0.5 g/L Lysozyme | |||
#*Prepared 2 mL 50 mM CaCl<sub>2</sub> (used our CaCl<sub>2</sub> calibration stock) | |||
#*Prepared 2 mL 500 μM CaCl<sub>2</sub> (used a CaCl<sub>2</sub> calibration dilution) | |||
#*Prepared 2 mL 50 mM NaCl (used our NaCl calibration stock) | |||
#*Prepared 1 mL 0.25 mM HCl using our 2.5 mM stored stock | |||
#*Cut a piece of 3500 MWCO flat dialysis tubing, wet it, and insert it in the 5-well dialysis chamber | |||
#*Clamp the dialysis chamber shut | |||
#*Filled one side of each well with 1 mL of our 0.5 g/L Lysozyme solution | |||
#**Transfered 1 mL to a plastic test tube using a pippetter, then | |||
#**Carefully filled each well with with a glass Pasteur pipette | |||
#*Filled the other side of the well with one of the following solutions | |||
#**HPLC water, 0.25 mM HCl, 50 mM CaCl<sub>2</sub>, 500 μM CaCl<sub>2</sub>, or 50 mM NaCl | |||
#**Each well has a different solution, which will be tested tomorrow | |||
#*Inserted top screws to prevent leaking/evaporation | |||
#*Tomorrow, UV-vis analysis will be done on all of the solutions from each well. | |||
#GPC will be conducted later | |||
#Ca<sup>2+</sup> calibration curve | #Ca<sup>2+</sup> calibration curve |
Revision as of 17:34, 1 October 2014
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ObjectiveCreated a create a Ca2+ calibration curve and began a new dialysis Description
The protocol above was adapted from Dr. Fox's Notebook. Data
In the figure below, the values of the potential were taken after the addition of ions. If the ion added first did not affect the value of the potential, the next ion was added to that same solution. As can be seen below, there is essentially no change in the potential value measured after the addition of any of the ions. This means that the presence of other ions has no effect on the potential reading of CaCl2. NotesThis area is for any observations or conclusions that you would like to note.
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