User:Michael S. Bible/Notebook/581/2014/08/29: Difference between revisions
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==Objective== | ==Objective== | ||
Completing film synthesis, starting new film synthesis, and making stock [http://en.wikipedia.org/wiki/Malachite_green Malachite green] solutions. | |||
==Description== | ==Description== | ||
# | # Completing film synthesis | ||
## Warm 150mL of Sodium Sulfate Solution (see below for details) at 80C for 15 minutes | |||
## Place film in Sodium Sulfate Solution (see below for details) at 80C for 1 hour with gentle stirring | |||
## Warm 150mL of 1% HCl at 80C for 15 minutes | |||
## Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring | |||
## Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring | |||
# Starting new film synthesis | |||
## Use [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/08/27|Dr. Hartings' Wednesday's Procedure]] to make new 10% clay-polyvinyl alcohol film | |||
### It was 10% by weight clay because we used 1g of PVA and 0.11g of clay | |||
I prepared two new polymer films using the following quantities of reactants: | |||
*PVA weight-1.0029g (Polymer only) | |||
*PVA weight-1.0038g (Polymer and clay) | |||
*Na montmorillonite-0.1105g | |||
# Malachite Green Solutions | |||
## We need to make the following solutions | |||
### 25mL 0.20ppm Malachite Green | |||
### 25mL 0.50ppm Malachite Green | |||
### 25mL 1.1ppm Malachite Green | |||
### 25mL 1.4ppm Malachite Green | |||
### 25mL 1.7ppm Malachite Green | |||
### 50mL 2.0ppm Malachite Green | |||
### 50mL 8.0ppm Malachite Green | |||
### 50mL 80.0ppm Malachite Green | |||
### 50mL 200.0ppm Malachite Green | |||
#### My group prepared the solutions for the 0.20ppm and 0.50ppm Malachite Green from a 200ppm stock solution. The rest of the solutions were prepared by other groups in the lab section. | |||
## Take a UV-Visible spectrum of the 0.2ppm to 8ppm standards. | |||
## Make a standard curve to determine the molar absorptivity of Malachite Green | |||
==Data== | ==Data== | ||
<u>Stock Solutions</u> | |||
# 0.2M Sodium Sulfate Solution | |||
## Andrew dissolved 22.034g of anhydrous Na<sub>2</sub>SO<sub>4</sub> in 1000mL of water | |||
## Andrew added 100mL of concentrated H<sub>2</sub>SO<sub>4</sub> to this solution | |||
## This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film | |||
# 1% wt. HCl Solution | |||
## Andrew added 10mL of concentrated HCl to 985mL of water | |||
## This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film | |||
# 0.2M Sodium Bicarbonate Solution | |||
## I dissolved 17.042g of NaHCO<sub>3</sub> in 1000mL of water | |||
## This solution will be used to neutralize the acid in your films | |||
==Notes== | ==Notes== |
Revision as of 19:55, 1 September 2014
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ObjectiveCompleting film synthesis, starting new film synthesis, and making stock Malachite green solutions. Description
I prepared two new polymer films using the following quantities of reactants:
DataStock Solutions
NotesThis area is for any observations or conclusions that you would like to note.
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