User:Michael S. Bible/Notebook/581/2014/10/01: Difference between revisions

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==Description==
==Description==
Put your description here
 
<u>Diffusion</u>
# Our group used a PVA film
# Jake, Mary, and James used a PVA/clay film
##We prepared the diffusion chambers in the following manner
# Set the film between the two chambers
# Added liquid to each chamber
## One chamber was filled with 10 mL water
## The other chamber was filled with 10 mL 20ppm Malachite green
# Withdraw 200uL from each chamber at the 5 minute mark and place in their own eppy tube
## NOTE: I want the groups to coordinate so that their 5 minutes don't overlap with one another.
## NOTE: We used a small volume cuvette
## NOTE: We took a 0 minute measurement for each.
## NOTE: We had to dilute the sample from our chamber with the 20ppm. We used a 1:2 dilution each time.  So, when we returned the sample, we only returned the non-diluted sample.
# We measured the UV-Vis spectrum for both samples and returned each to the chamber it originally came from
# We took measurements at the following times
## 6 minutes
## 17 minutes
## 30 minutes
## 1 hour
## 2 hours
## right before we left lab


==Data==
==Data==

Revision as of 06:53, 1 October 2014

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Objective

Put your objective here.

Description

Diffusion

  1. Our group used a PVA film
  2. Jake, Mary, and James used a PVA/clay film
    1. We prepared the diffusion chambers in the following manner
  3. Set the film between the two chambers
  4. Added liquid to each chamber
    1. One chamber was filled with 10 mL water
    2. The other chamber was filled with 10 mL 20ppm Malachite green
  5. Withdraw 200uL from each chamber at the 5 minute mark and place in their own eppy tube
    1. NOTE: I want the groups to coordinate so that their 5 minutes don't overlap with one another.
    2. NOTE: We used a small volume cuvette
    3. NOTE: We took a 0 minute measurement for each.
    4. NOTE: We had to dilute the sample from our chamber with the 20ppm. We used a 1:2 dilution each time. So, when we returned the sample, we only returned the non-diluted sample.
  6. We measured the UV-Vis spectrum for both samples and returned each to the chamber it originally came from
  7. We took measurements at the following times
    1. 6 minutes
    2. 17 minutes
    3. 30 minutes
    4. 1 hour
    5. 2 hours
    6. right before we left lab

Data

  • Add data and results here...

Notes

Add notes here.




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