User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/06: Difference between revisions
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* After 30 min. on ice, the cups were put in a heat block at 37C for 5 min. and followed by 5 min. on ice. | * After 30 min. on ice, the cups were put in a heat block at 37C for 5 min. and followed by 5 min. on ice. | ||
* To the cells, 800ul TY medium was added and incubated at 37C for 1 hour. | * To the cells, 800ul TY medium was added and incubated at 37C for 1 hour. | ||
* The cells were centrifuged for 1 min. at full speed and the cells were resuspended in 200μL TY-medium, and 100μL was plated on TY-Agar-Amp plates. The plates were stored at 37C o.n. | |||
* The | * The remaining 100ul was diluted 100x and 100μL was plated on TY-Agar-Amp plates and incubated o.n. at 37C. | ||
'''Inoculation of TY-medium | '''Inoculation of TY-medium for Glycerol Stocks''' | ||
* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10'' | * GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10'' |
Revision as of 00:36, 8 July 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
LabworkTransformation of E. coli TOP10 with BBa_J23109, BBa_J23100 and BBa_J23106
All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups.
Inoculation of TY-medium for Glycerol Stocks
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