User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/06: Difference between revisions
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== | ==Labwork== | ||
* | '''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106''' | ||
* BBa_J23109 iGEM plate 2, 2G | |||
* BBa_J23100 iGEM plate 1, 18C | |||
* BBa_J23106 iGEM plate 1, 18O | |||
All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups. | |||
* Four cups with 50ul competent ''E.coli TOP10'' cells were thawed on ice, and 2ul of dissolved plasmid was added. | |||
* After 30 min. on ice, the cups were put in a heat block at 37C for 5 min. and followed by 5 min. on ice. | |||
* To the cells, 800ul TY medium was added and incubated at 37C for 1 hour. | |||
* 100ul was plated on TY-Agar-Amp plates and incubated o.n. at 37C. | |||
* The remainer was centrifuged for 1 min. at full speed and the cells were resuspended in 100ul TY-medium, and plated on TY-Agar-Amp plated. The plates were stored at 37C o.n. | |||
'''Inoculation of TY-medium with Glycerol Stocks''' | |||
* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10'' | |||
* Terminator vector ? (2x) in ''E.coli TOP10'' | |||
* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n. | |||
* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n. | |||
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Revision as of 02:42, 7 July 2009
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LabworkTransformation of E. coli TOP10 with BBa_J23109, BBa_J23100 and BBa_J23106
All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups.
Inoculation of TY-medium with Glycerol Stocks
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