User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/08

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(Autocreate 2009/07/08 Entry for User:Michael_Verhoeven/Notebook/iGEM_2009_M._Verhoeven_Parts)
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'''Colonies grown for 30 hours'''
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From the transformed ''E. coli TOP10'' cells with [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 BBa_J23109], [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 BBa_J23106] three single colonies of each were chosen to plate anew on TY-agar-amp. plates. After hitting the colony, the cells were swiped in a single straight line, the tip heated and a new stripe through the old was made. This was repeated three times in total. The plates were stored at 37C over night.
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Colonies grown for 30 hours

From the transformed E. coli TOP10 cells with BBa_J23109, BBa_J23100 and BBa_J23106 three single colonies of each were chosen to plate anew on TY-agar-amp. plates. After hitting the colony, the cells were swiped in a single straight line, the tip heated and a new stripe through the old was made. This was repeated three times in total. The plates were stored at 37C over night.


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