User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/09

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(Autocreate 2009/07/09 Entry for User:Michael_Verhoeven/Notebook/iGEM_2009_M._Verhoeven_Parts)
Current revision (08:01, 9 July 2009) (view source)
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==Entry title==
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==Labwork==
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'''Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation'''
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* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 BBa_J23109] (labelled as no.1) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10''
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* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100] (labelled as no.2) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10'' (red colony)
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* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 BBa_J23106] (labelled as no.3) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10'' (red colony)
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* The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul). 
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* The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.
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Labwork

Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation

  • BBa_J23109 (labelled as no.1) vector BBa_J61002 in E.coli TOP10
  • BBa_J23100 (labelled as no.2) vector BBa_J61002 in E.coli TOP10 (red colony)
  • BBa_J23106 (labelled as no.3) vector BBa_J61002 in E.coli TOP10 (red colony)
  • The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul).
  • The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.




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