User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/09: Difference between revisions

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(Autocreate 2009/07/09 Entry for User:Michael_Verhoeven/Notebook/iGEM_2009_M._Verhoeven_Parts)
 
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==Entry title==
==Labwork==
* Insert content here...
 
'''Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation'''
 
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 BBa_J23109] (labelled as no.1) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10''
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100] (labelled as no.2) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10'' (red colony)
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 BBa_J23106] (labelled as no.3) vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] in ''E.coli TOP10'' (red colony)
* The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul). 
* The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.
 




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Revision as of 05:01, 9 July 2009

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Labwork

Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation

  • BBa_J23109 (labelled as no.1) vector BBa_J61002 in E.coli TOP10
  • BBa_J23100 (labelled as no.2) vector BBa_J61002 in E.coli TOP10 (red colony)
  • BBa_J23106 (labelled as no.3) vector BBa_J61002 in E.coli TOP10 (red colony)
  • The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul).
  • The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.
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