User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/08/31: Difference between revisions

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::x = 1.5 x 10<sup>-9</sup> mol / mL x 66776 g/mol = 1.00 x 10<sup>-3</sup>g
::x = 1.5 x 10<sup>-9</sup> mol / mL x 66776 g/mol = 1.00 x 10<sup>-3</sup>g


The solution is covered in aluminum foil and stored at room temperature overnight.
*The solution is covered in aluminum foil and stored at room temperature overnight.


==Data==
==Data==

Revision as of 06:12, 5 September 2011

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Objective

To use BSA (bovine serum albumin) to synthesize gold nanoparticles. BSA reduces Au3+ to Au 0 for the synthesis to occur.

Description

  • Add 0.0015 mM BSA and 0.25 mM of HAuCl4 to 10 mL of 50mM acetate buffer (pH 5.46) at room temperature. The ratio of Au to BSA is 167 for optimum results.
    • Note: distilled water used to rinse gold and BSA into mixture from weigh boats.
    • Four-place balance used to determine weights. Actual weights determined to be 0.0011g BSA and 0.0004g gold.
  • Heat mixture at 80°C for four hours (actual temperature varies closer to 70°C).
  • Using a UV-Vis Spectrophotometer, obtain spectra every 30 minutes, starting at t=0 minutes
  • Calculations:
Au
0.25mM Au = 0.00025 mol / L = 2.5 x 10-7 mol / mL
2.5 x 10-7 mol / mL = x / 10 mL
x = 2.5 x 10-6 mol / mL x 196.967 g/mol = 4.92 x 10-4g
BSA
0.0015mM BSA = 0.0000015 mol / L = 1.5 x 10-9 mol / mL
1.5 x 10-9 mol / mL = x / 10 mL
x = 1.5 x 10-9 mol / mL x 66776 g/mol = 1.00 x 10-3g
  • The solution is covered in aluminum foil and stored at room temperature overnight.

Data

  • Add data and results here...

Notes

The color of the mixture turned a very slight yellow over time, then returned to a clear color. The UV-Vis spectra were taken from 200-800nm using a 1mL quartz cuvette.



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