User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/03/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Au/BSA Mole Ratios</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 12: Line 12:


https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit
https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit
*To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.


==Description==
==Description==
# Add experimental record here. Include what, how, and why...
 
Effect of Au/BSA Ratio
 
# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
#* 90 Au/BSA
#* 110 Au/BSA
#* 130 Au/BSA
#* 150 Au/BSA
# Dilute to 1 mL with water.
# Place in oven at 80°C.
# Leave solutions in oven for 3.5 hr.
 
 
Effect of Tris Buffer on Protein Fibers
 
# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/02/15 |02/15/12]].
# Take UV-vis absorption data.
# Compare data to that obtained on [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/02/15 |02/15/12]], [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/02/21 |02/21/12]], and [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/02/28 |02/28/12]].


==Data==
==Data==
* Add data and results here...
* Add data and results here...


Revision as of 13:32, 6 March 2012

Au/BSA Mole Ratios <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

Experimental section for solution effects of refolding buffer:

https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit


  • To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.

Description

Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 90 Au/BSA
    • 110 Au/BSA
    • 130 Au/BSA
    • 150 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3.5 hr.


Effect of Tris Buffer on Protein Fibers

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/03/06&oldid=590138"