User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/03/06: Difference between revisions

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==Data==
==Data==


* Add data and results here...
The following graph shows the data obtained over the past 3 weeks for the effect of tris on a fiber solution over time.
 
[[Image:Fibers_%2B_tris_week_3.png|500px]]
 
[[Image:Week_3_zoom.png|500px]]


==Notes==
==Notes==

Revision as of 13:35, 6 March 2012

Au/BSA Mole Ratios <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Experimental section for solution effects of refolding buffer:

https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit


  • To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.

Description

Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 90 Au/BSA
    • 110 Au/BSA
    • 130 Au/BSA
    • 150 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3.5 hr.


Effect of Tris Buffer on Protein Fibers

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.

Data

The following graph shows the data obtained over the past 3 weeks for the effect of tris on a fiber solution over time.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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