User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/04/04: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microwave-Assisted Base Hydrolysis</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
==Objective==
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To test the effects of microwave-assisted base hydrolysis to determine the concentration of free BSA in solution.


==Description==
==Description==
# Add experimental record here. Include what, how, and why...
 
# Add 40 μL of BSA or AuNP solution (synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]])to 40 μL 4 M NaOH.
# Add 2μL 450 mM DTT.
# Heat in microwave for 1 min.
# Take UV-vis data.


==Data==
==Data==
* Add data and results here...
==Notes==
This area is for any observations or conclusions that you would like to note.


* Graph of UV-vis data:


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
[[Image:Absorbance vs wavelength 4-4-12.png]]


[[Category:Course]]
==Notes==
[[Category:Miscellaneous]]




Revision as of 09:34, 17 April 2012

Microwave-Assisted Base Hydrolysis <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To test the effects of microwave-assisted base hydrolysis to determine the concentration of free BSA in solution.

Description

  1. Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.
  2. Add 2μL 450 mM DTT.
  3. Heat in microwave for 1 min.
  4. Take UV-vis data.

Data

  • Graph of UV-vis data:

Notes


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