User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/04/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Ionic Strength Effects on Fibers</span> | ||
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==Objective== | ==Objective== | ||
To determine the ionic strength of 50 mM tris buffer (pH 10.1) on AuNP fibers. | |||
==Description== | ==Description== | ||
# Add | |||
# Centrifuge fiber solution made on [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/03/27 |03/27/12]] at 4500 rpm for 45 minutes. | |||
# Remove solution, leaving only supernatant. | |||
# Add 5 mL 50 mM tris (pH 10.1). | |||
# Centrifuge for 10 min at 13200 rpm. | |||
# Take UV-vis data. | |||
==Data== | ==Data== | ||
* Graph of absorbance vs wavelength for tris buffer added to AuNP fibers: | |||
[[Image:Absorbance vs wavelength magnified 4-10-12.png]] | |||
==Notes== | |||
Revision as of 09:37, 17 April 2012
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ObjectiveTo determine the ionic strength of 50 mM tris buffer (pH 10.1) on AuNP fibers. Description
Data
Notes |