User:Mike Barnkob: Difference between revisions

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I'm medical student from Denmark, with interests in microbiology, genetics and ways to engineer human cells.
==Information==


==Contact Info==
*Mike Barnkob
[[Image:OWWEmblem.png|thumb|right|Mike Barnkob (an artistic interpretation)]]
*Website: [http://www.mikebarnkob.dk www.mikebarnkob.dk].


*Mike Barnkob
==Protocols==
*Faculty of Health, University of Southern Denmark
 
*Email: [[Special:Emailuser/Mike Barnkob|Say hallo]].
Cloning
*Blog: [http://www.mikebarnkob.dk www.mikebarnkob.dk].
* Basics
** [[Mike_Barnkob:Protocols/Cloning/Solutions|Solutions]]
** [[Mike_Barnkob:Protocols/Cloning/Primer_Design|Primer design]]
** [[Mike_Barnkob:Protocols/Cloning/Gels|Gels]]
** [[Mike_Barnkob:Protocols/Cloning/Isolating_mRNA_from_spleen|Isolating mRNA from spleen]]
** [[Mike_Barnkob:Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]]
** [[Mike_Barnkob:Protocols/Cloning/PCR|PCR]]
* CRISPR / Cas9
** Designing sgRNA oligos
** [[Mike_Barnkob:Protocols/CRISPR/Cloning_oligos_into_backbone|Cloning oligos into backbone]]
** [[Mike_Barnkob:Protocols/CRISPR/Transforming_bacteria|Transforming bacteria with construct]]
** [[Mike_Barnkob:Protocols/CRISPR/Screening_for_inserts|Screening for inserts]]
* Lentiviral work
** Lentiviral production
** Infecting target cells
 
Bacterial work
* [[Mike_Barnkob:Protocols/Bacterial/Antibiotics|Prepare antibiotics]]
* [[Mike_Barnkob:Protocols/Bacterial/LB_agar_plates|LB agar plates]]
* [[Mike_Barnkob:Protocols/Bacterial/Streaking|Streaking plates]]
* [[Mike_Barnkob:Protocols/Bacterial/ON_liquid_culture|Inoculate overnight liquid culture]]
* [[Mike_Barnkob:Protocols/Bacterial/Glycerol_stock|Glycerol stock]]
* [[Mike_Barnkob:Protocols/Bacterial/Isolate DNA|Isolate plasmid DNA]]
* [[Mike_Barnkob:Protocols/Bacterial/Diagnostic_Restriction_Digest|Diagnostic Restriction Digest]]


==Education==
Immunology
* Flow cytometry
** [[Mike_Barnkob:Protocols/Immunology/Flow/General_notes|General notes]]
** [[Mike_Barnkob:Protocols/Immunology/Flow/Generic protocol|Generic protocol]]
** [[Mike_Barnkob:Protocols/Immunology/Flow/Preparing_beads|Preparing beads]]
* CFSE staining
* [[Mike_Barnkob:Protocols/Immunology/Enrichment_from_tumours|T cell enrichment from tumours]]
* [[Mike_Barnkob:Protocols/Immunology/Making_tetramer|Making tetramers]]


* 2012, MD, Faculty of Health, University of Southern Denmark
Tissue culture work
* 2009, B.Sc.med, Faculty of Health, University of Southern Denmark
* [[Mike_Barnkob:Protocols/Mediums|Media]]
* [[Mike_Barnkob:Protocols/Tissue_Culture/Thawing cells|Thawing cells]]
* Freezing down cells
* Split cells
* Count cells
* Harvest splenocytes
* Harvest BMDCs
* ''In vitro'' expansion of T cells
* Soft agar assay


==Research interests==
Visualization
* Long-time co-culture and visualization with live-dead marker
* [[Mike_Barnkob:Protocols/Visualization/Immunofluorescence_on_cell_lines|Immunofluorescence on cell lines]]


# Synthetic biology
Basics
# Cancer research
* [[Mike_Barnkob:Protocols/Formulars|Formulars]]
# Lab automation


==Projects==
==Projects==


'''Current:'''
I work on:
 
* T cell and cancer cell interactions
[[Mike_Barnkob/Projects/Liquid handling robot]]. An attempt to create a small liquid handling robot to do simple experiments with.
* Genetic manipulation of T cells


'''Past:'''
'''Past:'''


[http://2009.igem.org/Team:SDU-Denmark Bacto Bandage]. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).  
[[Mike_Barnkob:Projects/Liquid handling robot|Liquid handling robot]]. An attempt to create a small liquid handling robot to do simple experiments with.


==Publications==
[http://2009.igem.org/Team:SDU-Denmark Bacto Bandage]. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).

Latest revision as of 08:06, 20 July 2015

Information

Protocols

Cloning

Bacterial work

Immunology

Tissue culture work

  • Media
  • Thawing cells
  • Freezing down cells
  • Split cells
  • Count cells
  • Harvest splenocytes
  • Harvest BMDCs
  • In vitro expansion of T cells
  • Soft agar assay

Visualization

Basics

Projects

I work on:

  • T cell and cancer cell interactions
  • Genetic manipulation of T cells

Past:

Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.

Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).

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