User:Mike Barnkob: Difference between revisions
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==Protocols== | ==Protocols== | ||
Cloning | |||
* Basics | |||
** [[Mike_Barnkob:Protocols/Cloning/Solutions|Solutions]] | |||
** [[Mike_Barnkob:Protocols/Cloning/Primer_Design|Primer design]] | |||
** [[Mike_Barnkob:Protocols/Cloning/Gels|Gels]] | |||
** [[Mike_Barnkob:Protocols/Cloning/Isolating_mRNA_from_spleen|Isolating mRNA from spleen]] | |||
** [[Mike_Barnkob:Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]] | |||
** [[Mike_Barnkob:Protocols/Cloning/PCR|PCR]] | |||
* CRISPR / Cas9 | * CRISPR / Cas9 | ||
** Designing sgRNA oligos | ** Designing sgRNA oligos | ||
** Cloning oligos into backbone | ** [[Mike_Barnkob:Protocols/CRISPR/Cloning_oligos_into_backbone|Cloning oligos into backbone]] | ||
** Transforming bacteria with construct | ** [[Mike_Barnkob:Protocols/CRISPR/Transforming_bacteria|Transforming bacteria with construct]] | ||
** Screening for inserts | ** [[Mike_Barnkob:Protocols/CRISPR/Screening_for_inserts|Screening for inserts]] | ||
* Lentiviral work | * Lentiviral work | ||
** Lentiviral production | ** Lentiviral production | ||
| Line 17: | Line 24: | ||
Bacterial work | Bacterial work | ||
* | * [[Mike_Barnkob:Protocols/Bacterial/Antibiotics|Prepare antibiotics]] | ||
* | * [[Mike_Barnkob:Protocols/Bacterial/LB_agar_plates|LB agar plates]] | ||
* [[Mike_Barnkob:Protocols/Bacterial/Streaking|Streaking plates]] | |||
* [[Mike_Barnkob:Protocols/Bacterial/ON_liquid_culture|Inoculate overnight liquid culture]] | |||
* [[Mike_Barnkob:Protocols/Bacterial/Glycerol_stock|Glycerol stock]] | |||
* [[Mike_Barnkob:Protocols/Bacterial/Isolate DNA|Isolate plasmid DNA]] | |||
* [[Mike_Barnkob:Protocols/Bacterial/Diagnostic_Restriction_Digest|Diagnostic Restriction Digest]] | |||
Immunology | Immunology | ||
* Flow cytometry | * Flow cytometry | ||
** [[Mike_Barnkob:Protocols/Immunology/Flow/General_notes|General notes]] | |||
** [[Mike_Barnkob:Protocols/Immunology/Flow/Generic protocol|Generic protocol]] | |||
** [[Mike_Barnkob:Protocols/Immunology/Flow/Preparing_beads|Preparing beads]] | |||
* CFSE staining | * CFSE staining | ||
* [[Mike_Barnkob:Protocols/Immunology/Enrichment_from_tumours|T cell enrichment from tumours]] | |||
* [[Mike_Barnkob:Protocols/Immunology/Making_tetramer|Making tetramers]] | |||
Tissue culture work | Tissue culture work | ||
* [[Mike_Barnkob:Protocols/Mediums|Media]] | * [[Mike_Barnkob:Protocols/Mediums|Media]] | ||
* | * [[Mike_Barnkob:Protocols/Tissue_Culture/Thawing cells|Thawing cells]] | ||
* Freezing down cells | * Freezing down cells | ||
* Split cells | * Split cells | ||
| Line 37: | Line 51: | ||
* ''In vitro'' expansion of T cells | * ''In vitro'' expansion of T cells | ||
* Soft agar assay | * Soft agar assay | ||
Visualization | Visualization | ||
* Long-time co-culture and visualization with live-dead marker | * Long-time co-culture and visualization with live-dead marker | ||
* [[Mike_Barnkob:Protocols/Visualization/Immunofluorescence_on_cell_lines|Immunofluorescence on cell lines]] | |||
Basics | |||
* [[Mike_Barnkob:Protocols/Formulars|Formulars]] | |||
==Projects== | ==Projects== | ||
Latest revision as of 08:06, 20 July 2015
Information
- Mike Barnkob
- Website: www.mikebarnkob.dk.
Protocols
Cloning
- Basics
- CRISPR / Cas9
- Designing sgRNA oligos
- Cloning oligos into backbone
- Transforming bacteria with construct
- Screening for inserts
- Lentiviral work
- Lentiviral production
- Infecting target cells
Bacterial work
- Prepare antibiotics
- LB agar plates
- Streaking plates
- Inoculate overnight liquid culture
- Glycerol stock
- Isolate plasmid DNA
- Diagnostic Restriction Digest
Immunology
- Flow cytometry
- CFSE staining
- T cell enrichment from tumours
- Making tetramers
Tissue culture work
- Media
- Thawing cells
- Freezing down cells
- Split cells
- Count cells
- Harvest splenocytes
- Harvest BMDCs
- In vitro expansion of T cells
- Soft agar assay
Visualization
- Long-time co-culture and visualization with live-dead marker
- Immunofluorescence on cell lines
Basics
Projects
I work on:
- T cell and cancer cell interactions
- Genetic manipulation of T cells
Past:
Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.
Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).
