User:Mike Barnkob

Information

• Mike Barnkob
• Website: www.mikebarnkob.dk.

Protocols

Coloning

• CRISPR / Cas9
  • Designing sgRNA oligos
  • Cloning oligos into backbone
  • Transforming bacteria with construct
  • Screening for inserts
• Lentiviral work
  • Lentiviral production
  • Infecting target cells

Bacterial work

• Making LB agar plates

Immunology

• Flow cytometry
• CFSE staining

Tissue culture work

• Media
  • R10
  • D10
  • DMEM for 293TLA cells
• Thawing cells
• Freezing down cells
• Split cells
• Count cells
• Harvest splenocytes
• Harvest BMDCs
• In vitro expansion of T cells
• Soft agar assay

Mouse work

• Bleeding
• Harvesting bone-marrow DC's

Visualization

• Long-time co-culture and visualization with live-dead marker

Projects

I work on:

• T cell and cancer cell interactions
• Genetic manipulation of T cells

Past:

Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.

Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).