User:Mischa L Spiegel/Notebook/The effects of DMSP on angiogenesis/2015/07/03: Difference between revisions
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(Autocreate 2015/07/03 Entry for User:Mischa_L_Spiegel/Notebook/The_effects_of_DMSP_on_angiogenesis) |
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== | ==Day 4== | ||
* | * Stained cells from serial dilutions in 96 well plate with methylene blue and read at A610 | ||
* Found that cells of 16,000 and 8,000 did not grow well or adhere | |||
* Not much difference between 4,000; 2,000; 1,000 | |||
* Chose to begin with 2,000 for room to grow- for proliferation studies next week | |||
Thawed WT(U1)8 cells P13 in 10% DMSO, 90% serum and put into cell culture for proliferation assays next week | |||
Revision as of 08:35, 3 July 2015
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Day 4
Thawed WT(U1)8 cells P13 in 10% DMSO, 90% serum and put into cell culture for proliferation assays next week |