User:Mobeen Ashraf/Notebook/Chem-581/2012/11/02: Difference between revisions

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==Description==
==Description==


The following sulfate/porphyrin solutions were prepared and the absorbance was measured for each one between 200 and 800 nm by using UV/VIS instrument:
Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:


1) 1mL 50μM Na2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used


2) 1mL 50μM Fe2(SO4)3, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


3) 1mL 50μM FeSO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


4) 1mL 50μM H2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
* 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used


5) 1mL 50μM Al2(SO4)3
* 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.


==Data==
==Data==
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==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
[[Category:Course]]
[[Category:Miscellaneous]]


'''Note: The graph in the data section is a courtesy of Allison Alix.
'''




Revision as of 16:18, 30 November 2012

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Objective

  • Make 1:1 ratio of sulfate ions solution ( H2SO4, Fe2(SO4)3, FeSO4, Na2SO4, Al2(SO4)3).
  • Adsorption through films similar to clay films instead of clay :
    • 1.04 g Fe(II) Cl, 0.5 g Iron Sulfate, 1 film, and 40 ml H2O.
    • To precipitate iron oxide out of the solution NaOH ( 2.67 g in 10 ml H2O) was added drop wise with stirring.
    • The solution was then filtered using vacuum filtration and left to dry.
  • Add film with Porphyrin and glutaraldehyde to 1% solution of HCl and leave out until the next working day.

Description

Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:

  • 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.

Data

Notes

Note: The graph in the data section is a courtesy of Allison Alix.



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