User:Moira M. Esson/Notebook/CHEM-571/2013/08/28: Difference between revisions
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==Objectives== | ==Objectives== | ||
#Prepare a bovine serum albumin (referred to as BSA) and gold nanoparticle (referred to as AuNP) solution for later testing. | |||
#Prepare a sodium citrate- AuNP solution. | |||
#Determine the concentration of the prepared sodium citrate-AuNP solution using UV/vis. | |||
<br> | |||
==BSA-AuNP Preparation== | |||
*The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/08/28|Dr.Harting's AU-Biomaterials Design Lab Notebook]] and [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference]. | |||
<br> | |||
General Protocol: | |||
#Add 1mL 2.54mM HAuCl<sub>4</sub> solution prepared by Dr. Hartings to an 10mL volumetric flask. | |||
#Add 1.80mL 15.6μM BSA solution to the 10mL volumetric flask.(The concentration of gold in the solution is 90x that of the BSA) | |||
#Add 7.2mL deionized H<sub>2</sub>O to the 10mL volumetric flask. | |||
#Transfer the solution into a test tube. Cap the test tube with aluminum foil | |||
#Heat in oven at 80°C for 3hrs. | |||
#Store the solution in a blue falcon tube. | |||
'''Note''':Use caution when removing the test tube from oven. The test tube will be hot. | |||
<br> | |||
==Sodium citrate-AuNP solution Preparation== | |||
*The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/08/28|Dr.Harting's AU-Biomaterials Design Lab Notebook]] and [http://pubs.acs.org/doi/pdf/10.1021/ac0702084 this reference]. | |||
<br> | |||
General Protocol: | |||
#Add 50mL of the prepared 0.295mM HAuCl<sub>4</sub> solution to a clean 150mL beaker. | |||
#Heat this solution to a boil while stirring the solution with a magnetic stir bar. Keep the beaker covered with a watch glass while the solution is heating. | |||
#After the solution comes to a boil, add 1.5mL of 1%(w/v) sodium citrate(prepared by Dr. Harting's). | |||
#Cover the beaker with a watch glass and allow to boil and stir for another 40 minutes. | |||
#Remove the beaker from the hot plate and allow to cool to room temperature. Keep the watch glass on the top of the beaker until solution is cooled. | |||
#Measure the volume of the solution. | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Prepared citrate-AuNP particle solution''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Final volume(mL)||||9.5 | |||
|- | |||
| Final concentration citrate(M)||||0.0054 | |||
|- | |||
| Final concentration Au(M)||||0.0013 | |||
|} | |||
<br> | |||
==UV/vis analysis== | |||
Revision as of 19:26, 28 August 2013
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Objectives
BSA-AuNP Preparation
Note:Use caution when removing the test tube from oven. The test tube will be hot.
Sodium citrate-AuNP solution Preparation
UV/vis analysis |