User:Moira M. Esson/Notebook/CHEM-571/2013/08/28: Difference between revisions
From OpenWetWare
Line 53: | Line 53: | ||
*The maximum absorbance of the citrate-AuNP was 0.405 at 518-521nm. | *The maximum absorbance of the citrate-AuNP was 0.405 at 518-521nm. | ||
*This wavelength range did not correspond to the supporting reference. This may indicate that too little gold or the incorrect amount of the citrate-AuNP solution was added to the 1/10 dilution. Another dilution will be run during the next meeting in order to determine the concentration of the prepared citrate-AuNP solution and the size of the diameter of the Au particles in the solution. | *This wavelength range did not correspond to the supporting reference. This may indicate that too little gold or the incorrect amount of the citrate-AuNP solution was added to the 1/10 dilution. Another dilution will be run during the next meeting in order to determine the concentration of the prepared citrate-AuNP solution and the size of the diameter of the Au particles in the solution. | ||
*Although another spectrum will be obtained to determine the exact diameter size of the AuNP, according to [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf this reference] the diameter of the gold nanoparticles is less than 32nm. | |||
==Notes== | ==Notes== | ||
*After the addition of the citrate to the HAuCl<sub>4</sub> solution, the solution turned a light purple color. This color darkened during the 40min boiling process. Half way through the boiling process the solution turned a deep reddish purple color for approximately 5 min and then changed back to a dark purple color. | *After the addition of the citrate to the HAuCl<sub>4</sub> solution, the solution turned a light purple color. This color darkened during the 40min boiling process. Half way through the boiling process the solution turned a deep reddish purple color for approximately 5 min and then changed back to a dark purple color. |
Revision as of 17:15, 1 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||
Objectives
BSA-AuNP Preparation
Note:Use caution when removing the test tube from oven. The test tube will be hot.
Sodium citrate-AuNP solution Preparation
UV/vis analysis
Notes
|