User:Moira M. Esson/Notebook/CHEM-571/2013/09/03: Difference between revisions
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==Preparation of Inosine Stock Solutions== | ==Preparation of Inosine Stock Solutions== | ||
General Protocol | |||
#In a clean 25mL volumetric flask, add 0.1347g inosine. Fill the volumetric flask with deionized H<sub>2</sub>O up until the white line. | |||
#*'''Note''': To ensure the full addition of all of the inosine, a small amount of deionized H<sub>2</sub>O was placed on the weigh boat and a pipette was used to remove all of the H<sub>2</sub>O on the weigh boat.s | |||
#Cap the volumetric flask and shake the volumetric flask vigorously until the inosine is completely dissolved. | |||
#*'''Note''':The solution should be shaken vigorously for a minimum of 3 minutes. | |||
#Pour the solution into a 50mL falcon tube for later storage. | |||
#Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H<sub>2</sub>O. | |||
<br> | |||
==Notes== | ==Notes== |
Revision as of 12:05, 3 September 2013
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Objectives
Preparation of Adenosine Stock solutionGeneral Protocol
Preparation of Inosine Stock SolutionsGeneral Protocol
Notes
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