User:Moira M. Esson/Notebook/CHEM-571/2013/09/03: Difference between revisions
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'''Figure 1. Absorbance of Standard Adenosine Solutions''' | '''Figure 1. Absorbance of Standard Adenosine Solutions''' | ||
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[[Image: | [[Image:Correctedabsadenosine09042013rerunzem.png]] | ||
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*Maximum absorbance occurred at 260nm for each adenosine concentration | *Maximum absorbance occurred at 260nm for each adenosine concentration | ||
'''Figure 2. Calibration Curve of Adenosine Solutions''' | |||
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[[Image:Calibrationcurveadenosinezem09082013.png]] | |||
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'''Figure 3. Absorbance of Standard Inosine Solutions''' | |||
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[[Image:Correctedabsinosinezem09042013.png]] | |||
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*The maximum absorbance of each solution occurred at 249nm. | |||
'''Figure 4. Calibration Curve of Inosine Solutions''' | |||
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[[Image:Calibrationcurveinosinestdszem09082013.png]] | |||
==Notes== | ==Notes== | ||
*Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed | *Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed thoroughly with deionized H<sub>2</sub>O. | ||
*Adenosine samples of 1.0E-5M and 3.0E-5M were re-prepared and re-run because these points were outliers on our original calibration curve. | |||
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Revision as of 19:10, 8 September 2013
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Objectives
Preparation of Adenosine Stock solutionGeneral Protocol
Preparation of Inosine Stock SolutionsGeneral Protocol
UV/visFigure 1. Absorbance of Standard Adenosine Solutions
Figure 2. Calibration Curve of Adenosine Solutions
Figure 4. Calibration Curve of Inosine Solutions
Notes
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