Objectives
- Prepare adenosine solutions of various concentrations to determine the molar absorptivity of adenosine.
- Prepare inosine solutions of various concentrations to determine the molar absorptivity of inosine.
- Prepare calibration curves for adenosine and inosine.
==Preparation of Adenosine Stock solution
- In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H2O up until the white line.
- Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
- Note:The solution should be shaken vigorously for a minimum of 5 minutes.
- Pour the solution into a 50mL falcon tube for later storage.
- Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.
- Stock Solution Preparation for Adenosine
Total Mass [g]
|
Stock Volume [mL]
|
Concentration [M]
|
0.2006 |
50 |
0.015 |
|
- Serial Dilution of Adenosine Stock Solution
Dilution
|
'M1'[mol/L]
|
'V1' [mL]
|
'M2'[mol/L]
|
'V2' [mL]
|
1 |
0.015 |
0.1 |
1.5E-4 |
10 |
|
2 |
0.015 |
0.25 |
3.0E-5 |
10 |
|
3 |
1.5E-4 |
1.667 |
2.5E-5 |
10 |
|
4 |
1.5E-4 |
1.333 |
2.0E-5 |
10 |
|
5 |
1.5E-4 |
1.0 |
1.5E-5 |
10 |
|
6 |
1.5E-4 |
0.667 |
1.0E-5 |
10 |
|
7 |
1.5E-4 |
0.333 |
0.5E-5 |
10 |
|
8 |
1.5E-4 |
0.5 |
0.75E-5 |
10 |
|
|