User:Moira M. Esson/Notebook/CHEM-571/2013/09/04

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Objectives

  • Perform error analysis on the compiled data calibration curve.
    • Determine the standard deviation for the data points.
    • Determine the confidence interval for 90% and 95% confidence.
    • Determine if any data can be ruled out using a Q-test.
  • Determine the molar extinction coefficient for both adenosine and inosine.
  • Determine the concentration of an unknown adenosine sample(taken from Javier's group).
  • Perform Grubb's test on outlying peak of adenosine molar extinction coefficent graph.
  • Perform data analysis on molar extinction coefficient graphs(both adenosine and inosine).


Group calibration curve data

Figure 1. CHEM-571 Calibration Curve for Adenosine

  • The Grubb's test will be employed to determine whether the points that lie outside of the line of best fit are outliers


Figure 2. CHEM-571 Calibration Curve for Inosine

Table 1. Molar absorptivity for Adenosine and Inosine

Molar Absorptivity(Lmol-1cm-1) '
Adenosine 14025
Inosine 11007


Table 2. Standard deviation of Inosine Absorbance values

Comcentration(M) Standard Deviation
4.80E-05 0.10707708
4.00E-05 0.12254917
3.20E-05 0.0943149
2.40E-05 0.06671357
1.60E-05 0.04841797
8.00E-06 0.04748368


Table 3. Standard deviation of Adenosine Absorbance values

Concentration(M) Standard Deviation
3.00E-05 0.072537576
2.50E-05 0.045680412
2.00E-05 0.064123319
1.50E-05 0.056753854
1.00E-05 0.0313289
5.00E-06 0.046559639


Table 4. 95% Confidence Intervals for Adenosine Absorbance values

Concentration(M) Confidence Interval
3.00E-05 0.063580821
2.50E-05 0.04003991
2.00E-05 0.056205534
1.50E-05 0.049746033
1.00E-05 0.027460487
5.00E-06 0.040810573


Table 5. 90% Confidence Intervals for Adenosine Absorbance Values

Concentration(M) Confidence Interval
3.00E-05 0.053358707
2.50E-05 0.033602552
2.00E-05 0.047169171
1.50E-05 0.041748187
1.00E-05 0.023045567
5.00E-06 0.034249313


Table 6. 95% Confidence Intervals for Inosine Absorbance Values

Concentration(M) Confidence Interval
4.80E-05 0.093855474
4.00E-05 0.10741711
3.20E-05 0.082669136
2.40E-05 0.058475948
1.60E-05 0.042439442
8.00E-06 0.041620516


Table 7. 90% Confidence Intervals for Inosine Absorbance Values

Concentration(M) Confidence Interval
4.80E-05 0.078765997
4.00E-05 0.09014728
3.20E-05 0.069378126
2.40E-05 0.049074562
1.60E-05 0.035616303
8.00E-06 0.034929038


Unknown Adenosine Solution

Figure 3. Absorption Spectrum of Unknown Concentration

  • Using the molar extinction coefficient determined from the calibration curve above the concentration of the unknown is 4.7344E-5 M.



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