User:Moira M. Esson/Notebook/CHEM-571/2013/09/10: Difference between revisions
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*Note: The UV-vis spectra was taken with the blank automatically subtracted out. | *Note: The UV-vis spectra was taken with the blank automatically subtracted out. | ||
<br> | <br> | ||
'''Table 1. Preparation of ADA standard solutions''' | '''Table 1. Preparation of ADA standard solutions from 10mg/2mL stock solution''' | ||
<br> | <br> | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Number''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''ADA (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Buffer (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Coomassive Blue(μL)''' | |||
|- | |||
| 1||10||0.0056||0.794||200 | |||
|- | |||
| 2||9||0.005||0.795||200 | |||
|- | |||
| 3||7||0.0039||0.7961||200 | |||
|- | |||
| 4||5||0.0028||0.7972||200 | |||
|- | |||
| 5||3||0.0017||0.7983||200 | |||
|} | |||
<br> | <br> | ||
*Note: 1μg/mL was too small of a volume to prepare from the 10mg/mL stock. Another stock solution of 20μg/μL was prepared(with buffer and ADA from the stock solution) and a dilution to 1μg/μL was performed from the 20μg/μL solution. | |||
<br> | |||
'''Table 2. Preparation of 20μg/μL ADA standard solution''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''ADA (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Buffer(mL)''' | |||
|- | |||
| 0.0111||0.9889 | |||
|} | |||
<br> | |||
'''Table 3. Preparation of 1μg/μL ADA standard solution from 20μg/μL ADA standard solution''' | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample number''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/μL)''' | |||
| align="center" style="background:#f0f0f0;"|'''ADA (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Buffer(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Coomassive Blue(μL)''' | |||
|- | |||
| 6||1||0.05||0.75||200 | |||
|} | |||
<br> | |||
[[Image:AbsorbanceADAbradford09102013zem.png]] | [[Image:AbsorbanceADAbradford09102013zem.png]] | ||
<br> | <br> |
Revision as of 08:07, 14 September 2013
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Objectives
Bradford Assay General Protocol
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