User:Moira M. Esson/Notebook/CHEM-571/2013/09/11: Difference between revisions
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* Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | * Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | ||
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==Adenosine Deaminase Bradford assay== | ==Adenosine Deaminase Bradford assay== | ||
*All standard solutions were re-prepared using the procedure described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | *All standard solutions were re-prepared using the procedure described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | ||
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[[Image:AAcalibrationcurvechem57109112013zem.png]] | [[Image:AAcalibrationcurvechem57109112013zem.png]] | ||
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'''Figure 4. CHEM-571 Group calibration curve of gold nanoparticles''' | |||
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[[Image:GroupcalibrationcurveAAgoldchem571zem.png]] | |||
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'''Table 1. Grubb's test for prepared group calibration curve of gold standards''' | |||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Suspected Outliers(Abs)''' | |||
| align="center" style="background:#f0f0f0;"|'''G<sub>exp</sub>''' | |||
|- | |||
| 5||0.1164||1.288081628 | |||
|- | |||
| 10||0.2102||1.140265886 | |||
|- | |||
| 15||0.2856||1.216520053 | |||
|- | |||
| 20||0.4572||1.571958215 | |||
|- | |||
| 25||0.5377||1.029873242 | |||
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| 25||0.4619||1.014767716 | |||
|} | |||
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*With N=4, G<sub>critical</sub> at 95% is 1.46. The absorbance of 0.4572 for 20μg/mL can be discarded with 95% certainty. | |||
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'''Table 2. Concentration and atom number of gold in prepared solutions''' | |||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration gold(ug/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Atoms gold''' | |||
|- | |||
| BSA||0.015551764||4.77E+13 | |||
|- | |||
| Citrate-AuNP 1/10 dilution||0.006918789||2.11529E+13 | |||
|} | |||
Revision as of 10:32, 18 September 2013
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Objectives
Adenosine Deaminase Bradford assay
Atomic AbsorptionFigure 3. Calibration curve of gold nanoparticles
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