User:Moira M. Esson/Notebook/CHEM-571/2013/09/11: Difference between revisions
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== | ==Objectives== | ||
* | * Run Au-nanoparticles samples prepared [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]] on the AA. | ||
* Run BSA solution prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] on the AA. | |||
* Prepare a 1/10 dilution of the Au-citrate solution prepared [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] and run this sample on the AA. | |||
* Prepare calibration curve for Au-nanoparticle solutions and determine the concentration of Au in the BSA and citrate solutions. | |||
* Re-run bradford assay of adenosine deaminase that was unsuccessful on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | |||
* Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | |||
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==Adenosine Deaminase Bradford assay== | |||
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[[Image:ADAbradfordassap09112013zem.png]] | |||
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Revision as of 12:18, 11 September 2013
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Objectives
Adenosine Deaminase Bradford assay
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