User:Moira M. Esson/Notebook/CHEM-571/2013/09/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 17: Line 17:
<br>
<br>
[[Image:ADAbradfordassap09112013zem.png]]
[[Image:ADAbradfordassap09112013zem.png]]
<br>
[[Image:Calibrationcurvebradfordassay09112013zem.png]]
<br>
<br>
    
    

Revision as of 12:48, 11 September 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Run Au-nanoparticles samples prepared 09/10/2013 on the AA.
  • Run BSA solution prepared on 08/28/2013 on the AA.
  • Prepare a 1/10 dilution of the Au-citrate solution prepared 08/28/2013 and run this sample on the AA.
  • Prepare calibration curve for Au-nanoparticle solutions and determine the concentration of Au in the BSA and citrate solutions.
  • Re-run bradford assay of adenosine deaminase that was unsuccessful on 09/10/2013.
  • Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on 08/28/2013.


Adenosine Deaminase Bradford assay






Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Moira_M._Esson/Notebook/CHEM-571/2013/09/11&oldid=727042"