User:Moira M. Esson/Notebook/CHEM-571/2013/09/17: Difference between revisions
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== | ==Objectives== | ||
* | * Find endpoints of HRP (sodium dithionite will reduce and potassium ferrocyanide will oxidize).All buffers are degassed (bubbled nitrogen through for 3 hours)---endpoints of oxidation and reduction of HRP. look for 100% oxidized and 100% reduced. | ||
*Add very carefully and very slowly and then just let it sit for about 5 minutes before take the sample. Need blank spectra(buffer) of the cuvette | |||
*solutions HRP that are 1mL 10μM when have 17.25μM starting. | |||
Revision as of 09:21, 17 September 2013
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