User:Moira M. Esson/Notebook/CHEM-571/2013/09/17

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/09/17 Entry for User:Moira_M._Esson/Notebook/CHEM-571)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objectives==
-
* Insert content here...
+
* Find endpoints of HRP (sodium dithionite will reduce and potassium ferrocyanide will oxidize).All buffers are degassed (bubbled nitrogen through for 3 hours)---endpoints of oxidation and reduction of HRP. look for 100% oxidized and 100% reduced.
 +
*Add very carefully and very slowly and then just let it sit for about 5 minutes before take the sample. Need blank spectra(buffer) of the cuvette
 +
*solutions HRP that are 1mL 10μM when have 17.25μM starting.  

Revision as of 12:21, 17 September 2013

Project name Main project page
Previous entry      Next entry

Objectives

  • Find endpoints of HRP (sodium dithionite will reduce and potassium ferrocyanide will oxidize).All buffers are degassed (bubbled nitrogen through for 3 hours)---endpoints of oxidation and reduction of HRP. look for 100% oxidized and 100% reduced.
  • Add very carefully and very slowly and then just let it sit for about 5 minutes before take the sample. Need blank spectra(buffer) of the cuvette
  • solutions HRP that are 1mL 10μM when have 17.25μM starting.



Personal tools