User:Moira M. Esson/Notebook/CHEM-571/2013/09/24: Difference between revisions
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##*Take 10μL of the diluted sample and add 10μL of SDS-PAGE buffer. | ##*Take 10μL of the diluted sample and add 10μL of SDS-PAGE buffer. | ||
##*Store prepared samples in the fridge | ##*Store prepared samples in the fridge | ||
##UV-vis sample preparation | |||
##*Remove 0.75mL of sample(either pepsin or pepstatin) and add 0.75mL perchloric acid | |||
#Repeat step 4 for two hours. | #Repeat step 4 for two hours. | ||
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==UV-vis== | |||
Figure 1. Absorbance spectra of hemoglobin with pepsin. | |||
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[[Image:Pepsin09242013zem.png]] | |||
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Figure 2. Absorbance spectra of hemoglobin with 20μM pepstatin | |||
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Revision as of 12:50, 24 September 2013
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Objectives
General ProtocolThe following protocol was derived from this reference.
UV-visFigure 1. Absorbance spectra of hemoglobin with pepsin.
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