User:Moira M. Esson/Notebook/CHEM-571/2013/09/25: Difference between revisions
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#Develop stain of the gel | #Develop stain of the gel | ||
#*Place gel in fixant solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes | #*Place gel in fixant solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes | ||
#*Place gel in Stain Solution (0.025% (w/v) Coomassie Blue, 10% acetic acid, 90% water) for 1 hour | |||
#*Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | |||
#Repeat last part of step 4 twice. | |||
<br> | <br> | ||
Table 1. Sample set up of electrophoresis gel | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Well Numbers''' | | align="center" style="background:#f0f0f0;"|'''Well Numbers''' |
Revision as of 10:22, 25 September 2013
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Objectives
Electrophoresis
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