User:Moira M. Esson/Notebook/CHEM-571/2013/09/25: Difference between revisions
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#Run electrophoresis for 30 minutes at 200V | #Run electrophoresis for 30 minutes at 200V | ||
#Develop stain of the gel | #Develop stain of the gel | ||
#*Place gel in fixant solution (40% methanol, 10% acetic acid, 50% water) for | #*Place gel in fixant solution (40% methanol, 10% acetic acid, 50% water) for 20 minutes to stop the proteins from migrating further | ||
#*Place gel in Stain Solution (0.025% (w/v) Coomassie Blue, 10% acetic acid, 90% water) for 1 hour | #*Place gel in Stain Solution (0.025% (w/v) Coomassie Blue, 10% acetic acid, 90% water) for 1 hour | ||
#*Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | #*Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes |
Revision as of 10:43, 25 September 2013
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Objectives
Electrophoresis
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