User:Moira M. Esson/Notebook/CHEM-571/2013/09/25: Difference between revisions
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Figure 1. SDS-PAGE Gel | |||
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[[Image:FLUBBER.jpg]] | |||
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==UV-vis== | ==UV-vis== | ||
Figure | Figure 2. Corrected Absorbance Spectra of Pepsin with Hemoglobin | ||
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[[Image:Hemopepsin09251013zem.png]] | [[Image:Hemopepsin09251013zem.png]] | ||
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*Note: For the 2hr sample some pieces of protein may have been present when running the sample. | *Note: For the 2hr sample some pieces of protein may have been present when running the sample. | ||
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Figure | Figure 3. Corrected Absorbance Spectra of 20μM Pepstatin with Hemoglobin | ||
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[[Image:Pepstathemogl09252013zem.png]] | [[Image:Pepstathemogl09252013zem.png]] | ||
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==Notes== | ==Notes== | ||
*For both of the UV-vis spectra, there is no observable trend for the absorbance(the absorbance does not increase or decrease consistently with time). This may be due to the inclusion of protein in some of the sample runs. Another trial of these samples will be performed | *For both of the UV-vis spectra, there is no observable trend for the absorbance(the absorbance does not increase or decrease consistently with time). This may be due to the inclusion of protein in some of the sample runs. Another trial of these samples will be performed | ||
*Placed the gel in 10mLs of fixing solutions. Actually ended up dying the gel for 1hour and 15mins. | |||
Revision as of 17:34, 7 October 2013
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Objectives
Electrophoresis
UV-visFigure 2. Corrected Absorbance Spectra of Pepsin with Hemoglobin
Notes
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