User:Moira M. Esson/Notebook/CHEM-571/2013/10/01: Difference between revisions
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== | ==Objectives== | ||
* | # Monitor the kinetics and yield of horseradish peroxidase catalyzed oxidation of luminol | ||
## Measure UV-Vis absorbance of luminol, HRP, and Luminol+HRP+H<sub>2</sub>O<sub>2</sub> | |||
## Monitor chemiluminscence of luminol oxidation using stop-flow techniques | |||
## Measure kinetics of reaction using stop-flow techniques and absorbance | |||
<br> | |||
==UV-vis== | |||
Stock solutions prepared before class: | |||
#Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8 | |||
#Buffer: 5mM Tris pH=8 | |||
#HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | |||
*Performed 20% dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution). | |||
**Make a 10mL stock solution of 20% dilute luminol stock solution. | |||
Revision as of 10:19, 1 October 2013
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Objectives
UV-visStock solutions prepared before class:
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