User:Moira M. Esson/Notebook/CHEM-571/2013/10/01

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==Entry title==
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==Objectives==
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# Monitor the kinetics and yield of horseradish peroxidase catalyzed oxidation of luminol
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## Measure UV-Vis absorbance of luminol, HRP, and Luminol+HRP+H<sub>2</sub>O<sub>2</sub>
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## Monitor chemiluminscence of luminol oxidation using stop-flow techniques
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## Measure kinetics of reaction using stop-flow techniques and absorbance
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<br>
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==UV-vis==
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Stock solutions prepared before class:
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#Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8
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#Buffer: 5mM Tris pH=8
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#HRP: 1.7μM HRP in 50mL 5mM Tris pH=8
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*Performed 20% dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution).
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**Make a 10mL stock solution of 20% dilute luminol stock solution.
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Revision as of 13:19, 1 October 2013

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Objectives

  1. Monitor the kinetics and yield of horseradish peroxidase catalyzed oxidation of luminol
    1. Measure UV-Vis absorbance of luminol, HRP, and Luminol+HRP+H2O2
    2. Monitor chemiluminscence of luminol oxidation using stop-flow techniques
    3. Measure kinetics of reaction using stop-flow techniques and absorbance


UV-vis

Stock solutions prepared before class:

  1. Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8
  2. Buffer: 5mM Tris pH=8
  3. HRP: 1.7μM HRP in 50mL 5mM Tris pH=8
  • Performed 20% dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution).
    • Make a 10mL stock solution of 20% dilute luminol stock solution.



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