User:Moira M. Esson/Notebook/CHEM-571/2013/10/01: Difference between revisions
From OpenWetWare
(Autocreate 2013/10/01 Entry for User:Moira_M._Esson/Notebook/CHEM-571) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objectives== | ||
* | # Monitor the kinetics and yield of horseradish peroxidase catalyzed oxidation of luminol | ||
## Measure UV-Vis absorbance of luminol, HRP, and Luminol+HRP+H<sub>2</sub>O<sub>2</sub> | |||
## Monitor chemiluminscence of luminol oxidation using stop-flow techniques | |||
## Measure kinetics of reaction using stop-flow techniques and absorbance | |||
<br> | |||
==UV-vis== | |||
Stock solutions prepared before class: | |||
#Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8 | |||
#Buffer: 5mM Tris pH=8 | |||
#HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | |||
*Performed 20% dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution). | |||
**Make a 10mL stock solution of 20% dilute luminol stock solution. | |||
Revision as of 10:19, 1 October 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
UV-visStock solutions prepared before class:
| |
