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Project name
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Objectives
- Monitor the kinetics and yield of horseradish peroxidase catalyzed oxidation of luminol
- Measure UV-Vis absorbance of luminol, HRP, and Luminol+HRP+H2O2
- Monitor chemiluminscence of luminol oxidation using stop-flow techniques
- Measure kinetics of reaction using stop-flow techniques and absorbance
UV-vis
Stock solutions prepared before class:
- Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8
- Buffer: 5mM Tris pH=8
- HRP: 1.7μM HRP in 50mL 5mM Tris pH=8
- Performed 20% dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution).
- Make a 10mL stock solution of 20% dilute luminol stock solution.
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