User:Moira M. Esson/Notebook/CHEM-571/2013/10/08: Difference between revisions
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'''Sample Preparation''': | '''Sample Preparation''': | ||
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Table 1. Preparation of 0.0123M stock solution | Table 1. Preparation of 0.0123M stock solution | ||
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| Volume of buffer(mL)||2.9902 | | Volume of buffer(mL)||2.9902 | ||
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'''UV-vis''': | |||
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Figure 1. Corrected Absorbance spectra of the conversion of adenosine to inosine by ADA | |||
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[[Image:Adakinetics10082013zem.png]] | |||
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*Using the molar absorptivity of inosine and adenosine determined on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/03|2013/09/03]], the conversion of adenosine to inosine can be represented as concentrations.The molar absorptivity of adenosine and inosine was also determined for all wavelengths. | |||
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Table 3. Molar Absorptivity of Adenosine at 260nm and Inosine at 250nm | |||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wavelength''' | |||
| align="center" style="background:#f0f0f0;"|'''Adenosine''' | |||
| align="center" style="background:#f0f0f0;"|'''Inosine''' | |||
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| 250||13333||11937.5 | |||
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| 260||16866.7||7312.5 | |||
|} | |||
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Figure 2. Monitoring concentration versus time of the conversion of adenosine to inosine using ADA as a catalyst. | |||
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[[Image:ConcvstimeadentoinoADAcatalystzem.png]] | |||
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*There appears to be values in which negative concentrations appear for both inosine and adenosine. For the first 60 seconds, no ADA had been added. As such, the negative values should be present for the inosine. For both inosine and adenosine, at 105 seconds, a large amount of inosine was produced and a negative concentration of adenosine was present. This may represent the fastest point in the reaction of the conversion of adenosine to inosine. | |||
Revision as of 11:37, 15 October 2013
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Objectives
KineticsGeneral Protocol:
UV-vis:
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