User:Moira M. Esson/Notebook/CHEM-571/2013/10/22: Difference between revisions
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==Objectives== | ==Objectives== | ||
#Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]]. | #Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]]. | ||
# | #Synthesize Pepsin-AuNPs and HRP-AuNPs for catalytic activity testing | ||
#Determine the absorbance of all starting materials used for solution preparation (gold and HRP) | |||
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==HRP-AuNP and Pepsin-AuNP== | ==HRP-AuNP and Pepsin-AuNP== | ||
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*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | |||
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Table 1. Preparation of Protein-AuNP solutions | Table 1. Preparation of Protein-AuNP solutions | ||
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| 210||0.496031746||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709 | | 210||0.496031746||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709 | ||
|} | |} | ||
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*The varying ratios will be used to determine the ideal concentrations for the preparation of fibers of unraveled proteins. | |||
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==UV/vis== | |||
*Absorbance of BSA-AuNPs was determined for future comparison of the activity of protein-AuNPs. | |||
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Figure 1. Absorbance spectra of a 1/10 dilution of BSA-AuNPs | |||
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[[Image:BSAAuNPsdilutionzem.png]] | |||
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*Absorbance of all starting materials used for synthesis of AuNPs today for future comparison | |||
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Figure 2. Absorbance spectra of a 1/10 dilution of 2.52mM gold in water | |||
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[[Image:Golddilutionzem.png]] | |||
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Figure 3. Absorbance spectra of 1/2 dilution of 15.3μM HRP | |||
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[[Image:HalfdilutedHRP10222013zem.png]] | |||
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Figure 4. Absorbance spectrum of 1/10 dilution of 15.6μM pepsin | |||
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[[Image:Pepsindilutionzem.png]] | |||
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*No absorbance peak was observed for pepsin. This was expected due to the lack of color of the pepsin stock solution. | |||
==Notes== | |||
*All of the solutions prepared had a yellowish tint | |||
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Revision as of 06:57, 28 October 2013
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Objectives
HRP-AuNP and Pepsin-AuNP
UV/vis
Notes
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