User:Moira M. Esson/Notebook/CHEM-571/2013/11/05: Difference between revisions
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#Transfer ~4mL of sample from test tubes into a 10mL falcon tube. | #Transfer ~4mL of sample from test tubes into a 10mL falcon tube. | ||
#Centrifuge ~4mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced. | #Centrifuge ~4mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced. | ||
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL). | #Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/23|09/23/2013]]. | ||
#Run samples on AA. | |||
#Prepare calibration curve and prepare a graph showing the concentration of Au vs Au:Protein ratio | |||
<br> | |||
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)''' | |||
|- | |||
| 10||0.1||9.9 | |||
|- | |||
| 20||0.2||9.8 | |||
|- | |||
| 30||0.3||9.7 | |||
|- | |||
| 40||0.4||9.6 | |||
|- | |||
| 50||0.5||9.5 | |||
|} | |||
Revision as of 12:14, 5 November 2013
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Objectives
AA spectroscopyGeneral Protocol:
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