User:Moira M. Esson/Notebook/CHEM-571/2013/11/05: Difference between revisions

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==Objectives==
==Objectives==
* Analyze protein-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/23|10/23/2013]] and [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/29|10/29/2013]] using Atomic Absorption spectroscopy
* Analyze protein-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/22|10/22/2013]] and [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/23|10/23/2013]] using Atomic Absorption spectroscopy
<br>
==AA spectroscopy==
'''General Protocol:'''
#Transfer ~4mL of sample from test tubes into a 10mL falcon tube.
#Centrifuge ~4mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL).
 
 




Revision as of 12:01, 5 November 2013

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Objectives


AA spectroscopy

General Protocol:

  1. Transfer ~4mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge ~4mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL).




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